Uniform and dense nano-spherical HA/Ag composite coatings were prepared by a pulse electrochemical deposition method on biomedical titanium surfaces. The coatings were characterized by XRD and SEM. Escherichia coli and Staphylococcus albus were utilized to test the antibacterial ability of the coatings. The cytocompatibility of composite coatings was evaluated by culturing osteoblast MC3T3-E1 on the coatings. The results demonstrate that Ag uniformly distribute in the composite coatings without agglomeration. The coatings have good antibacterial effects on the two types of bacteria. However, they have a better antibacterial effect on Escherichia coli of 100% than on Staphylococcus albus of near 100%. The MC3T3-E1 cell culturing results indicate that the MC3T3-E1 osteoblast has good adhesion and spreading on HA/Ag composite coatings. It is concluded that HA/Ag composite coatings prepared by pulse electrochemical deposition have good antibacterial property and cytocompatibility