To enhance the fracture toughness of TiB2 ceramic film, Ti/TiB2 bionic multilayers was formed by intervention of period Ti layer which was abstracted into a soft layer according to the structural characteristics of nacre. The improved indentation method was proposed to characterize the fracture toughness (KIC) of the Ti/TiB2 bionic multilayers. The results show that the KIC of the multilayers strongly depends on the modulation ratios (Λ, the ratio of TiB2 to Ti thickness, is denoted tTiB2:tTi) and modulation period (T). With the increase of modulation ratio, the KIC of the multilayers increases first and gets a maximum of2.68 MPa