TNZS, TiO2/TNZS and HA/TNZS bio-medical materials were prepared by powder metallurgy comebining high-energy milling with cold pressed sintering. Histocompatibility in vitro of the three TNZS-based materials were studied. The results show that: three materials have no cytotoxicity, meeting toxicity safety requirements of implant materials; CPIR on the surface of TiO2/TNZS and HA/TNZS for 1 d, 3 d, 5 d and 7 d have significantly lower than that of TNZS group, showing that the addition of TiO2 and HA can improve the speed of cell proliferation on the surface of TNZS material and have better induction of proliferation of osteoblasts in vitro; the distribution of osteoblasts attached to the surface of TiO2/TNZS has more uniform; pseudopodium extension of osteoblasts attached to the surface of three materials are in good condition.