The microstructure evolution and the change of Burgers orientation relationship between α and β phase of TC17 titanium alloy with initial lamellar microstructure were analyzed and researched by EBSD. The results shown that dynamic recrystallization of β phase was occured during hot deformation process. Meanwhile, the dynamic globularization of α phase contained the formation of subboundary and the fragmentation of α phase. The fragmentized α phase was eventually transformed to globularized particle. Meanwhile, the strict Burgers orientation relationship between α and β phase was destroyed, which was due to the activation of prismatic slip system and othe slip or twin system.