In order to prepare longer TiO2 nano-tube, Ti sheet was anodic oxidized under direct voltage of 20V in solution of 0.5(wt)％NaF＋glycerin for 7h, in the temperature of 0℃,20℃ and 40℃ respectively. The results of SEM show that the length of the TiO2 nano-tube are 1.034μm,2.601μm and 5.152μm for 0℃,20℃ and 40℃ respectively. The results of pH test show that diffusion coefficient of solution increases with temperature increasing, diffusion of H+ speed up near the Ti sheet, so the rate of pH reduction slow down. In the temperature of 40℃, in solution of 0.5(wt)％NaF＋glycerin, TiO2 nano-tube can grow to about 10μm for 12h, and surface ratio achieves 208 m2.g-1.