The high temperature flow behavior of TC31 titanium alloy at the 840~960℃ 0.0001~0.1s_^-1. and the effects of the deformation temperature, strain rate and strain on flow stress and microstructure were studied, respectively. the Arrhenius constitutive equation of TC31 titanium alloy was established and verified. The results show that when the temperature is lower than 880°C and the strain rate is higher than 0.01s_^-1, the material exhibits obvious dynamic softening. When the temperature is higher than 920°C and the strain rate is lower than 0.001s_^-1, the coarse grain leads to flow stress increasing. It is indicated that the strain, strain rate and deformation temperature all have different effects on grain size, shape and phase content. In addition, it is confirmed that the strain-corrected Arrhenius constitutive equation has higher prediction accuracy, and its MSE is 2.443 and the R value is 0.9675.