TA2 titanium substrate was etched by oxalic acid, hydrochloride acid and sulfuric acid, respectively, under the condition of the same acidity and temperature. The effects of various acids treatment on the properties of Ti substrate and the as-prepared electrodes were investigated. The results indicate that the mass loss rate of the titanium substrate treated by oxide acid and sulfuric acid are higher than that of the substrate treated by hydrochloride acid. The OM and SEM images show that the titanium substrate treated by oxide acid has the best surface morphology after the etching process, with the fine and uniform scores. The accelerated test life of the dimensional stable anode prepared from the titanium substrate treated by oxide acid is the longest among the three types’ electrodes. The oxygen evolution potentials of the three types’ electrodes are nearly the same, which means that the acid treatment has a slight influence on the oxygen evolution potential of the electrodes