Low cycle fatigue (LCF) properties of a nickel-based single crystal (SX) superalloy DD10 with different orientation deviation angles from the [001] axis was investigated at 760 °C and with invariable total strain amplitude of 1.4%. Results reveal that the LCF behavior of DD10 exhibited a strong orientation-dependence. The fatigue life decreased apparently with the increment of the orientation deviation angle. For specimens with the same orientation deviation angle from the [001] axis, those on the [001]-[011] boundary exhibited a longer fatigue life than ones on the [001]-[11] boundary. It was found that the orientation-dependence of fatigue life stems from the difference of accumulated plastic deformation per cycle. On the other hand, both of total stress amplitude and plastic strain amplitude showed a reverse orientation-dependence compared with the fatigue life. However, the orientation deviation showed no influence on the model of fatigue crack initiation and subsequently propagation.