Ti6Al4V titanium alloy was prepared by selective laser melting technology. The effects of different forming surfaces (XOY, XOZ) and different loads (20N, 40N, 60N, 80N) on the friction and wear properties of Ti6Al4V alloy were studied. The friction and wear properties of different forming surfaces were evaluated by friction coefficient (COF) combined with wear volume loss, and the wear track morphology and wear mechanism were characterized by optical microscope (OM) and three-dimensional profile measuring instrument. The results show that compared to the XOZ surface, the wear volume of the XOY surface is reduced by 0.27×10-5mm3 when the normal load is 20N, and the average friction coefficient is smaller. However, when the load is greater than 20N, the wear volume and average friction coefficient of XOY surface are larger than those of XOZ surface. The depth and width of the plough groove of the worn track increase with the increase of the load. The plough groove exhibits obvious peeling behavior, and there is a blocky "adhesive" distributed on the track, causing adhesive wear and oxidative wear. From the aspects of friction coefficient, wear volume and micro-morphology, it is quantitatively reflected that XOZ surface of Ti6Al4V alloy formed by SLM has better friction and wear performance than XOY surface, and XOY surface shows more wear resistance only under low load.