The effect of Ti-6Al-4V alloy powder recycling on the properties of powder for electron beam 3D printing was studied. The results show that with the increase of the number of cycles,the mass of the fine powder in the old powder decreases,the adhesion between the particles increases,the particle size distribution gathers to the coarse particles,the friction resistance of particles on the powder bed decreases,the compressibility increases,and the block residue also increases; the recycling of powder has little effect on the profile accuracy of the components,and the relative compactness decreases. Adding new powder can improve the bad performance of old powder,and the degree of improvement is related to the quantity of new powder.